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Fig. 2 | Rice

Fig. 2

From: Unique Glutelin Expression Patterns and Seed Endosperm Structure Facilitate Glutelin Accumulation in Polyploid Rice Seed

Fig. 2

Immunoblot analyses of seven glutelin species. The protein extracts derived from mature rice seeds in 9311 and A3 (harvested in 2019) were separated by SDS-PAGE (4%–20% gradient), and analyzed by immunoblotting using anti-GluA-1, anti-GluA-2, anti-GluB-1, anti-GluB-2, anti-GluB-4/5, anti-GluC-1, and anti-GluD-1 antibodies. Immune bands of glutelin acidic subunit were scanned and quantified using Image J software. Different lowercase letters indicate significant differences (p < 0.05) as determined by one-way ANOVA and Tukey’s test. Each lane contained rice powder of similar weight. Black triangles represent pro-glutelin and glutelin acidic subunit. β-tubulin was used as a loading control. Three independent experiments were performed and data are shown

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