Skip to main content
Rice
Fig. 8 | Rice

Fig. 8

From: Comparisons within the Rice GA 2-Oxidase Gene Family Revealed Three Dominant Paralogs and a Functional Attenuated Gene that Led to the Identification of Four Amino Acid Variants Associated with GA Deactivation Capability

Fig. 8

Comparisons of OsGA2ox7 and OsGA2ox3 3-D protein structures and sequence alignment to show the conserved amino acids between clades A and B and the phenotypes of WT-OX, 3R-OX, 3P-OX and 3RP-OX transgenic rice plants. a Simulated protein structure of OsGA2ox7 and published protein structure of OsGA2ox3 for comparison. The loop regions contain clade A-specific C186 and C194, and their corresponding amino acids R191 and P201 in OsGA2ox7 show different structures. The 3-D protein structures were visualized by using PyMOL (Schrodinger 2015). b Detailed consensus amino acid sequence alignment of the loop region between motif 9 and motif 10 distinguished by two conserved Cs (highlighted with green color) in clade A from other variants (highlighted with blue color) in clade B. c Phenotypic comparison of WT-OX, 3R-OX, 3P-OX and 3RP-OX transgenic rice plants. A representative overexpression T0 transgenic plant with the average RPH from each overexpression line is shown. Values are means ± SE (n = 5). d Statistical comparisons of the length of the main culms (including panicles and internodes) from each overexpression line. Values are means ± SE (n = 5). The significant differences using Student’s t-test are indicated by *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page