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Rice
Fig. 1 | Rice

Fig. 1

From: Comparisons within the Rice GA 2-Oxidase Gene Family Revealed Three Dominant Paralogs and a Functional Attenuated Gene that Led to the Identification of Four Amino Acid Variants Associated with GA Deactivation Capability

Fig. 1

Phenotypes and expression characterization of the T-DNA activation-tagged mutants for OsGA2ox1, 2, 4, 7, and 8. The phenotypes and relative plant height (RPH %) of T-DNA activation-tagged mutants (with various genotypes (W/W, segregated WT; T/W, heterozygous; T/T, homozygous)) compared with those of nontransgenic plants (NT) and the RT-PCR analysis results of target gene activation are shown. The Actin gene was used as a control. The T-DNA insertion sites (orange triangles) relative to the ATG start codon of their target genes are shown in the graphic adjacent to the images. a The T-DNA mutant M36548 (an OsGA2ox1ACT mutant), with the T-DNA inserted 5.9 kb upstream from OsGA2ox1, is shown. b The T-DNA mutant M43852 (an OsGA2ox2ACT mutant), with the T-DNA inserted 12.5 kb upstream from OsGA2ox2, is shown. c The T-DNA mutant M96803 (an OsGA2ox4ACT mutant), with the T-DNA inserted 2 kb upstream from OsGA2ox4, is shown. d The T-DNA mutant M66925 (an OsGA2ox7ACT mutant), with the T-DNA inserted 19 kb upstream from OsGA2ox7, is shown. e The T-DNA mutant M61685 (an OsGA2ox8ACT mutant), with the T-DNA inserted 15.5 kb downstream from OsGA2ox8, is shown. f RPH comparison of the homozygous plants of the above T-DNA activation-tagged mutants. Values are means ± SE (n = 10)

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