Fig. 1

Identification of PSL50 by IMBR strategy. a Phenotype of WT and psl50 at the mature stage. Scale bar = 20 cm. b Identification of target mutation of psl50 by IMBR strategy. Red dot indicates the causative mutation responsible for the psl50 phenotype. c Initial mapping of PSL50 and verification of target mutation in PSL50. d Deletion and insertion mutation at the target site of 1st exon in three representative knockout lines generated by CRISPR/Cas9 in ‘Kitaake’ background. The Cas9–2, Cas9–5 and Cas9–12 lines are homozygous mutants carrying a 1-bp deletion, a 2-bp deletion and a 1-bp insertion on both homochromosomes, respectively. e Phenotype of three representative T₀ knockout lines at the heading stage. Insets display magnified views of flag leaves. Empty vector represents transgenic Kitaake plant transformed with CRISPR/Cas9 empty vector. Scale bar = 20 cm