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Fig. 7 | Rice

Fig. 7

From: A Single Nucleotide Substitution of GSAM Gene Causes Massive Accumulation of Glutamate 1-Semialdehyde and Yellow Leaf Phenotype in Rice

Fig. 7

Expression analyses of related genes by qRT-PCR. a Expression pattern of OsGSAM gene. The total RNA was extracted from different tissues, including root (R), leaf blade (L), stem (S), leaf sheath (LS) and young panicle (P), of the wild type at seedling stage and booting stage, respectively. Rice actin 1 gene was used as an internal control. Error bars represent the SDs of three independent experiments. b and c Comparison of expression levels of the genes associated with tetrapyrrole biosynthesis and photosynthesis, respectively, between ys53 and its wild at seedling stage. The total RNA was extracted from 4-week-old seedlings. The relative mRNA amount of each gene was normalized to Actin 1. The expression level of each gene in the wild type was set to 1.0, and those in ys53 mutant were calculated accordingly. Error bars represent the SDs of three independent experiments. Asterisks indicate statistically significant differences compared with the wild type at P < 0.05

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