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Rice
Fig. 4 | Rice

Fig. 4

From: A Single Nucleotide Substitution of GSAM Gene Causes Massive Accumulation of Glutamate 1-Semialdehyde and Yellow Leaf Phenotype in Rice

Fig. 4

Determination of GSA and ALA content and ALA-synthesizing activity in ys53 and its wild type. a Total content of GSA and ALA in rice leaves at seedling stage. Leaves were harvested from normal growing seedlings in the paddy field on sunny afternoons, quick freezed in liquid nitrogen and used for determination of total content of GSA and ALA. b ALA-synthesizing activity of rice seedlings. After 1 week of dark culture, the yellow seedlings of ys53 and its wild type were cultured under light condition (100 μmol m− 2 s− 1) for 7 h in LA (levulinic acid) solution, and then the measurement of GSA and ALA was performed. Error bars represent the SDs of three independent experiments. * indicates statistically significant difference between ys53 and its wild type at P < 0.05

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