Xa7, a Small Orphan Gene Harboring Promoter Trap for AvrXa7, Leads to the Durable Resistance to Xanthomonas oryzae Pv. oryzae

Rice

Table 1 Complementary Transgenic Plants Generated in this Study

Constructs	Recipient varieties	HTP-resistant T₀ plants ^a	*Xoo*-resistant T₀ plants ^b
S1AE6	IR24	22	16
S1AG3	IR24	10	0
S2CD8	IR24	6	2
S1BA3	IR24	11	5
S1BA3	ZH11	33	11
S1BA3	TP309	15	2
CG52–1300	ZH11	14	4
CG52–1300	TP309	21	8
S2BD2	ZH11	44	13
S2BD2	TP309	39	17
S1AD4	IR24	6	0
P_PR1:Xa7:T_nos	TP309	28	21

^a The number of transgenic plants regenerated from hygromycin selection culture
^b The number of transgenic plants resistant to Xoo strain PXO86. To test resistance, five to ten of the uppermost fully expanded leaves of each transgenic plant were inoculated using the leaf tip-clipping method at the booting stage. The plants were scored as resistant or susceptible based on the average lesion length at two weeks after inoculation