Fig. 3

Phenotypes of HLH1- and HLH2-knockout lines in XQZ background. a Knockout of HLH1 caused loss of purple stigma in XQZ. Bar, 2 mm b Apiculus color changed from purple to straw-white in HLH2 knockout lines of XQZ. Bars, 2 mm. c Sequence alignment between XQZ and HLH1- and HLH2-knockout lines. + and - signs indicate base insertions (in blue) and deletion (by dashes), respectively, relative to XQZ. Gray boxes denote coding sequences of HLH1 and HLH2, and blue boxes are the untranslated regions. hlh1–1, HLH1-transgenic knockout line. hlh1–1-1and hlh1–1-2, two individuals of hlh1–1-transgenic line. hlh2–1 and hlh2–2, independent HLH2-transgenic knockout lines