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Fig. 4 | Rice

Fig. 4

From: RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice

Fig. 4

Protein-protein interaction between OsLIL3 and OsPSY2. (a), Yeast two hybrid assays. OsLIL3 and LHC domains were fused with Gal DNA-binding domain (BD). OsPSY2 was fused with Gal DNA-activation domain (AD). The two other combinations OsLIL3/empty (pGADT7 vector) and empty (pGBKT7 vector)/OsPSY2 were included as negative controls. Yeast cells containing various vector combinations were grown on DDO plates (SD –Leu –Trp) for co-transformation selection. Yeast cells showing growth and blue color on high selection TDO/X/A plates (SD-His-Leu-Trp + X-alfa-Gal + Aureobasidin A) confirmed positive interactions between the two indicated proteins. (b), OsLIL3-FLAG coimmunoprecipitated with OsPSY2-HA. Total protein was purified from tobacco leaves expressing OsLIL3-FLAG with or without OsPSY2-HA and immunoprecipitated with an anti-Flag or anti-HA antibody. The presence of OsLIL3-FLAG, and OsPSY2-HA in the complex was determined by immunoblot analysis with Flag anti-bodies and anti-HA anti-bodies respectively. (c), BiFC-visualized interactions between OsLIL3 and OsPSY2 in N. benthamiana leaves. Abbreviations: YN, YFPN. YC, YFPC. Chl, chlorophyll autofluorescence. BF, bright field. All bars = 5 μm

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