Fig. 5

Generation of knockout mutants for OsGI, PhyB and Hd1 with CRISPR strategy. a Comparison of expression of OsPRR37 among mutants ghd7, osgi, hd1, phyB, and control plant ZH11. Expression level of OsPRR37 in leaves collected 2.5 h after dawn from 40-d-old plants under controlled LD conditions were analyzed by qRT-PCR and shown as mean ± SD of three replicates. Rice ubiquitin gene (Os02g0161900) was used for normalization. **, P < 0.01, t-test. b-d Mutation details of genetic materials of OsGI (b), PhyB (c) and Hd1 (d). Black bars represent the exons, and solid line before, between and after the black bars represents the 5′ un-transcribed region, intron and 3′ un-transcribed region, respectively. Letters in the blue box is the PAM sequence. Blue letters indicate the targets sequence of indicated gene. Red letters indicate the mutation details. e-h Phenotype of whole plants (e-g); and main panicles (h) of indicated genetic materials. Scale bar, 25 cm in e-g and 5 cm in h. i-k Genetic analyses between OsPRR37 and OsGI (i), PhyB (j) and Hd1 (k). Data represent mean ± SD, n = 16. Different letters indicate significant differences at P < 0.05, Duncan’s test