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Rice
Fig. 7 | Rice

Fig. 7

From: Rice FLOURY SHRUNKEN ENDOSPERM 5 Encodes a Putative Plant Organelle RNA Recognition Protein that Is Required for cis-Splicing of Mitochondrial nad4 Intron 1

Fig. 7

Mitochondrial function and structure were disrupted in fse5 mutant cells. a Mitochondrial complexes separated by BN-PAGE and stained with Coomassie brilliant blue (CBB) (left) and in-gel NADH dehydrogenase activity of complex I (arrow) (right). b and c Measurement of ATP contents (b) and respiration rates (c). The values are the means ± SDs. **, P < 0.01, Student’s t test. d Mitochondria in developing endosperm cells of the WT and fse5 seeds at 9 DAF. Bars, 0.5 μm. e Expression of AOX-encoding genes (AOX1a, AOX1b and AOX1c) and alternative NADH dehydrogenase-encoding genes (NDA1, NDA2, NDB1, NDB2, NDB3, and NDC1) in nine-day-old seedlings. Specific primers were used for qRT-PCR (Toda et al. 2012; Li et al. 2013). The data are based on three biological replicates. OsActin1 was used as an internal control for data normalization. The values are the means ± SDs

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