Fig. 3
From: Efficient Gene Stacking in Rice Using the GAANTRY System
Representative phenotypes observed in the GAANTRY 11-stack T1 rice plants. a Hygromycin sensitive wildtype Nipponbare (WT, left) and resistant (11-stack right) seedlings 7 days post germination. b Measured Renilla luciferase activity for 11-stack leaf samples. c Firefly luciferase activity for 11-stack leaf samples. The average background levels of activity detectable in wildtype leaf extracts is shown with the dashed line at 1.0 × 103 units. d β-glucuronidase activity detected in green tissues and not in roots in a histochemically stained 7-day old 11-stack seedling. e Finale herbicide sensitivity in wildtype (left) and tolerance in 11-stack (right) leaves. f Observed green fluorescence localized in the root tips of a 11-stack rice plant. g The phenotypes of a wildtype (left) and 11-stack (right) seedlings germinated on media containing hygromycin and paromomycin antibiotics as well as glufosinate and glyphosate herbicides