Fig. 1
From: Efficient Gene Stacking in Rice Using the GAANTRY System
Composition of the 5-stack T -DNA. Diagram of an Agrobacterium rhizogenes virulence plasmid containing the 5-stack T-DNA. Cargo sequences, including the promoter, gene coding sequence and transcription terminator, are displayed as a colored arrow showing the orientation of transcription or a dark blue rectangle (TBS insulator). The T-DNA right border (RB) and left border (LB) locations are shown in green. Abbreviations are as follows: hptII, hygromycin phosphotransferase 2, Rluc (Renilla luciferase), TBS: Transformation Booster Sequence, Fluc (Firefly luciferase) and GUSPlus (β-glucuronidase encoding reporter gene). The gentamicin bacterial resistance marker (GmR) is shown outside the RB region of the T-DNA, within the native Agrobacterium virulence plasmid (pRi)