Fig. 5
From: Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease
IDD3, IDD13, and LPA1 form a complex to regulate PIN1a transcription. a A transient expression assay was conducted by co-transfection with p35S:IDD3, p35S:IDD13, p35S:LPA1, p35S:IDD3 + p35S:LPA1, p35S:IDD13 + p35S:LPA1 and the vector expressing the GUS under the control of native (pPIN1a) PIN1a promoters in protoplast cells. The luciferase gene driven by the 35S promoter was used as an internal control to normalize the GUS expression. Error bars represent the mean ± SE (n = 6). Different letters indicate significant differences at P < 0.05. b IDD3-HA + IDD13-Myc, IDD3-HA + IDD13-Myc + LPA1-GFP, or IDD3-HA + empty vector were transformed into tobacco leaves using Agrobacterium-mediated transformation. HA antibody-immunoprecipitated proteins were analyzed using western blot analysis with the Myc antibody. IDD3-HA, IDD13-Myc, and LPA1-GFP levels were analyzed by a western blot using HA, Myc, and GFP antibodies, respectively