Fig. 4
From: Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease
IDD3 and IDD13 bind and activate the PIN1a promoter. a IDD3-GFP and IDD13-GFP were detected in the lateral roots. GFP signal and bright field are shown in the left and right, respectively. Bars = 20 μm. b Schematic diagram indicating the location of the putative IDD-binding motif (gray circle) within 1.5 kb of the PIN1a promoter and the probes (P) used for chromatin immunoprecipitation (ChIP) assays. c Relative ratios of immunoprecipitated DNA to input DNA were determined by qPCR. Input DNA was used to normalize the data. –Ab or + Ab: green fluorescent protein (GFP) antibody. Error bars represent the mean ± SE (n = 3). d An electrophoretic mobility-shift assay (EMSA) was conducted to evaluate GST-IDD3 and GST-IDD13 affinities to P2 and mutated probe mP2. e A transient expression assay was conducted by co-transfection with p35S:IDD3 or p35S:IDD13 and each of the vectors expressing GUS under the control of native (pPIN1a) and IDD-binding motif-mutated (mpPIN1a) PIN1a promoters in protoplast cells. The luciferase gene driven by the 35S promoter was used as an internal control to normalize GUS expression. Error bars represent the mean ± SE (n = 6). Different letters indicate significant differences at P < 0.05