Fig. 5
From: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing
Application of a multiplexed iSTU-CRISPR/Cas12a system in rice. a Schematic illustration of the multiplexed iSTU-CRISPR/Cas12a expression system. b Gel electrophoresis and Sanger sequencing results of iSTU-CRISPR/Cas12a (inO) induced gene deletions at OsDEP1 and OsROC5 loci. Left, detection of targeted deletions by PCR. Deletion percentage was quantified by ImageJ. Right, Sanger sequencing results that further validated the deletions. c Sanger sequencing of one T0 line that carried mutations at both target sites by multiplexed genome editing