Fig. 3
From: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing
Application of a multiplexed iSTU-CRISPR/Cas9 system in rice. a Schematic illustration of a multiplexed iSTU-CRISPR/Cas9 (inO::tRNA) system based on tRNA processing. b The RFLP analysis of iSTU-CRISPR/Cas9 (inO::tRNA) multiplexing at OsPDS and OsDEP1 target sites. The insertion and deletion (InDel) percentage was quantified by ImageJ. c Sanger sequencing of three T0 lines that carried mutations at both target sites by the inO::tRNA based multiplex system. d Sanger sequencing of two T0 lines that carried mutations at both target sites by the inO::tRNA based multiplex system