Fig. 2

Comparison of the spikelet epidermal cells between WT and mis2. (a-d) Scanning electron micrographs (SEM) for the outer surfaces of lemma (a, b) and palea (c, d) of WT (a, c) and mis2 (b, d). CL and CW indicate epidermal cell length and cell width orientation, respectively. Bars = 100 μm. (e, f) The average length and width of the epidermal cells. (n = 10). (g) Comparisons of the calculated epidermal cell number of the lemma and palea in the grain-length (GL) and grain-width (GW) direction, respectively (n = 10). (h-k) SEM for the inner surfaces of lemma (h, i) and palea (j, k) of WT and mis2. Bars = 100 μm. (l, m) Cross sections of WT and mis2 spikelet hull. Bar = 50 μm. WLC, wart-like cells; SC, silicified cells; OPC, outer parenchyma cells; IPC, inner parenchyma cells. * P < 0.05 and ** P < 0.01 by Student’s t test. Data are given as mean ± SD (n = 20)