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Table 2 Deletions out of frame (Out-frame), deletions that maintain reading frame (In-Frame) generated using SpCAS9, LbCPF1 and BE_nCAS9D10A expressed as % of total mutant alleles identified. A total of 60, 30 and 22 mutants alleles were identified for SpCAS9, LbCPF1 and BE_nCAS9D10A respectively (see also Fig. 3)

From: Beyond Seek and Destroy: how to Generate Allelic Series Using Genome Editing Tools

 

InFrame

OutFrame

SpCAS9

10% (6)

90% (56)

LbCPF1

43% (13)

57% (17)

BE_CAS9

95% (21)

5% (1)