Fig. 7
From: OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice
OsMS188 directly regulates the expression of CYP703A3, CYP704B2, OsPKS2, OsPKS1, DPW and ABCG15. a QRT-PCR analysis of CYP703A3, CYP704B2, OsPKS2, OsPKS1, DPW and ABCG15 expression in the inflorescences in WT, osms188 and tdr backgrounds. b-g EMSAs showing that OsMS188 can bind to the fragments of the CYP703A3, CYP704B2, OsPKS2, OsPKS1, DPW and ABCG15 promoter regions. MBP-tagged proteins were mixed with a biotin-labelled probe, and unlabelled probes were used as competitors. The arrowhead indicates a shifted band. h Transient dual-luciferase assays were performed in Arabidopsis leaf protoplasts. A p35S::NOS vector was used as a negative control. p35s::OsMS188nos and p35s::TDRnos were transformed into protoplasts together with reporter plasmids. Three replicates were assessed, and the Y-axis shows the ratio of luciferase/Renilla. The errors bars indicate the SDs