Identification of Novel Alleles of the Rice Blast-Resistance Gene Pi9 through Sequence-Based Allele Mining

Rice

Table 4 Disease responses of Pigm, Pi2, Pizt, Pi9 and Pi9 alleles honor plants to M. oryzae isolates

Gene	Varieties	Resistance ratio	Resistance level
None	LTH	0.00%	8.15 ± 0.77
None	J23B	0.00%	6.22 ± 1.19
Pigm	GM4H	91.20%	2.43 ± 1.82
Pi2	C101 A51	67.60%	3.81 ± 1.84
Pi9	75–1-127	94.10%	2.71 ± 1.14
Pizt	DY1H	58.80%	4.16 ± 2.70
Pi9-Type1	Heo Trang	26.50%	5.10 ± 2.14
Pi9-Type2	IR64	23.50%	5.62 ± 1.70
Pi9-Type3 ^a	HC1H	85.30%	2.88 ± 2.09
Pi9-Type4 ^a	PIIB	88.20%	2.01 ± 1.31
Pi9-Type5 ^a	GD-1S	100.00%	1.79 ± 0.72
Pi9-Type5-Pi2	CT 18664–9–18-1-3-2	70.60%	3.15 ± 2.00
Pi9-Type5-Piz-t	ASD 18	67.60%	3.56 ± 1.76
Pi9-Type6 ^a	YD4038	91.20%	2.03 ± 1.25
Pi9-Type7	KAUKKYI ANI	61.80%	4.04 ± 2.45
Pi9-Type8	DY1H	58.80%	4.16 ± 2.70
Pi9-Type9 ^a	THAVALU	100.00%	1.79 ± 0.72
Pi9-Type10 ^a	ZWH210	94.10%	2.07 ± 1.59
Pi9-Type11 ^a	R03138	88.20%	2.75 ± 1.58
Pi9-Type12	JP-5	79.40%	2.72 ± 1.64
Pi9-Type13	ZD5H	55.90%	4.43 ± 2.36

^aWhen Resistance ratio was greater than 85% and Resistance level was less than 2, NILs of allele materials were done
Resistance ratio, the proportion above MR inoculated with 34 M. oryzae isolates; Resistance level, the average level inoculated with 34 M. oryzae isolates. None, no functional Pi9 allele; Pi9-Type5 has three different type Pi9-Type5, Pi9-Type5-Pi2 and Pi9-Type5-Piz-t. Donors of Pi9-Type5-Pi2 and Pi9-Type5-Piz-t carried Pi2 and Piz-t genes, respectively. Donors of Pi9-Type4 carried Pigm. Donors of Pi9-Type8 carried Piz-t