Fig. 3
From: RGG1, Involved in the Cytokinin Regulatory Pathway, Controls Grain Size in Rice
Overexpression of RGG1 has multiple effects on agronomic traits. a Targeted mutation of RGG1 using the CRISPR/Cas9 system generated two mutants (NIP-rgg1–1 and NIP-rgg1–2), which were confirmed by sequencing. b Relative expression levels of NIP and two RGG1 overexpression lines (NIP-OE1 and NIP-OE2). OsActin was selected as the internal control. c Plant morphology of NIP, NIP-rgg1–1, NIP-rgg1–2, NIP-OE1 and NIP-OE2 at the mature stage. Scale bar, 10 cm. d Panicle phenotypes of NIP, NIP-rgg1–1, NIP-rgg1–2, NIP-OE1 and NIP-OE2. Scale bar, 5 cm. e Grain sizes of NIP, NIP-rgg1–1, NIP-rgg1–2, NIP-OE1 and NIP-OE2. Scale bar, 1 cm. f-m Comparisons among NIP, the mutants and the OE lines with respect to f plant height; g panicle length; h tiller number per plant; i grain number per panicle; j grain length; k grain width; l 1000-grain weight; and m grain yield per plant. The data are given as the mean ± SD (n ≥ 20). Different letters indicate significant differences ranked by the LSD test (P < 0.05)