Fig. 1
From: RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice
Characterization of the rice osacbp6 mutant. a T-DNA insertion in OsACBP6, and primer locations used for PCR genotyping. Black and grey boxes indicate coding and untranslated regions, respectively. b PCR genotyping of osacbp6. Primer combinations ML2066/ML2067 (top gel) and 2717LB/ML2067 (bottom gel) in PCR to identify osacbp6 homozygous mutant. c Semi-quantitative RT-PCR showing the knockout of OsACBP6 in osacbp6. ACTIN1 served as a control. d Growth of wild type (WT, Oryza sativa var. japonica cv. Dongjin) and osacbp6. Images from left to right represent 5-, 7-day-old seedlings grown in water, and 21-day-old soil-grown seedling. Bars, 1 cm. e The primary root length (left) and shoot length (right) comparison between WT and osacbp6. Primary root reduction of osacbp6 is shown in the middle. Values are mean ± SD (n = 25). f Comparison of the leaf length of 21-day-old soil-grown seedlings between WT and osacbp6. Values are mean ± SD (n = 5). Asterisks indicate significant differences between WT and osacbp6 as evaluated by Student’s t-tests: ***P < 0.001