Fig. 8
Oat-like rice phenotypes and statistics of OsMADS1Olr-overexpressing transgenic plants. a Grain shape and phenotypes of OsMADS1Olr-overexpressing plants (OE-2 and OE-10) with similar Oat-like rice phenotypes in comparison with grains of the wild-type plant (NIP). Arrowheads indicate conjugated twin brown rice. b Relative expression of the OsMADS1 in 12 DAF grains of wild-type and OsMADS1Olr-overexpressing plants. c Seed setting rate of wild-type and OsMADS1Olr-overexpressing plants. d-f Chromatogram of cDNA fragments spanning the mutation site in OsMADS1Olr or the corresponding wild-type site in OsMADS1 from RT-PCR amplification from NIP (d), OE-2 (e) and OE-10 (f) with a designed OsMADS1-RT primer pair (Additional file 1: Table S5). Red arrowheads indicate the signal peak of A at the mutation site in OsMADS1Olr cDNA derived from Oat-like rice, and blue arrowheads indicate the signal peak of G at the corresponding wild-type site in OsMADS1 cDNA derived from NIP. g Comparison of grain length, grain width, grain thickness, brown rice length, brown rice width, brown rice thickness, lemma length, palea length, lemma width, and palea width between wild-type and OsMADS1Olr-overexpressing plants. h-k Comparison of grain length-to-width ratio (h), brown rice length-to-width ratio (i), 1000-grain weigh (j) and 1000-brown rice weight (k) between wild-type and OsMADS1Olr-overexpressing plants. Bar: (a) 5 mm. Data presented are mean values ± SDs [n = 3 replicates in b, j and k; n = 12 replicates in c; n = 50 in g-i.]. Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.001