Fig. 7
Tissue-specific RNA expression pattern of the OsMADS1 and OsMADS1Olr. a Relative expression levels of the OsMADS1 and OsMADS1Olr in NIP and Oat-like rice, respectively. Samples from panicles, floral organs and grains at representative developmental stages were analyzed. P1, 0–3 mm panicles; P2, 3–15 mm panicles; P3, 15–50 mm panicles; P4, 50–100 mm panicles; P5, 100–150 mm panicles; P6, 150–200 mm panicles; P7, panicles longer than 200 mm; L1, lemmas of spikelets from 100 to 150 mm panicles; L2, lemmas of spikelets from 150 to 200 mm panicles; L3, lemmas of spikelets from panicles longer than 200 mm; Pa1, paleae of spikelets from 100 to 150 mm panicles; Pa2, paleae of spikelets from 150 to 200 mm panicles; Pa3, paleae of spikelets from panicles longer than 200 mm; St, stamens of spikelets before anthesis from panicles longer than 200 mm; Pi, pistils of spikelets before anthesis from panicles longer than 200 mm; S1, 1 DAF pistils and hulls; S3, 3 DAF grains; S6, 6 DAF grains; S9, 9 DAF grains; S12, 12 DAF grains; S15, 15 DAF grains; S18, 18 DAF grains; S21, 21 DAF grains. DAF, Days After Fertilization. The value of OsActin mRNA was used as an internal control for data normalization, and the expression level of OsMADS1 in S1 (1 DAF pistils and hulls) of NIP were set as 1.0. Values are means ± SDs of three replicates. b-n Histochemical staining analysis of GUS expression driven by OsMADS1 promoter in transgenic grains at representative developmental stages. b and c A mature spikelet (b) and hull of a mature spikelet (c). d A 6 DAF grain. e-g, j and m Brown rice. h, k and o The transverse section of brown rice. i, l and n Longitudinal section of brown rice. Bars: (b-n) 1 mm