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Table 3 Details of nks_bad2 gene based SSR marker for aroma

From: Marker Assisted Development and Characterization of Herbicide Tolerant Near Isogenic Lines of a Mega Basmati Rice Variety, “Pusa Basmati 1121”

Marker

Primer Sequences

Tm

PB1121 Allele

Robin Allele

Position (Mb)

nks_bad2

F: GGTTGCATTTACTGGGAGTTATG

58 °C

82 bp

90 bp

Gene based

R: TCCACAGAAATTTGGAAACAAA