Fig. 1

Map-based cloning of PGL14.aPGL14 localizes to the short arm on chromosome 11 between RM26076 and RM26079, and is narrowed down to a 39.5 kb region covering the bacterial artificial chromosome clones AC116949 and AC138196. The 39.5 kb region contains 7 putative ORFs, the black box indicates LOC_Os11g05552, gray boxes indicate the other ORFs. LOC_Os11g05552 consists of 15 exons and 14 introns indicated as blank boxes and lines, respectively. The black arrow indicates the point mutation (G278A) at 1st intron splicing site. PTsF and PTsR are the forward and reverse primers for PCR analysis in (b). F and R are forward and reverse primers for qRT-PCR analysis; b RT-PCR shows different transcripts from WT and pgl14. The genomic DNA was used as a control