Fig. 1From: Proteome-Wide Analyses Reveal the Diverse Functions of Lysine 2-Hydroxyisobutyrylation in Oryza sativaWorkflow for large-scale identification of Khib sites in rice. a Western blotting analysis of total proteins in rice seedling leaves. The gels for SDS-PAGE were stained with Coomassie Blue. b Workflow for identification of Khib modification in rice. Total proteins extracted from seedling leaves were digested to peptides by trypsin. Peptides bearing Khib sites were affinity enriched with a specific Khib antibody and subsequently analyzed by LC-MS/MS.Back to article page