Fig. 6
Splicing analysis of chloroplast intron-containing transcripts in the wild type and slc1 mutant leaves at the third-leaf stage. a RT-PCR analysis of 18 intron-containing genes in the wild type and slc1 mutant leaves. U, Unspliced transcripts; S, Spliced transcripts. b Splicing efficiency analysis of chloroplast intron-containing transcripts in the wild type and slc1 mutant leaves. psbA was used as internal control, and relative transcript levels were analyzed using the 2-ΔΔCT method (Livak and Schmittgen 2001). Three biological replicates were performed. Values indicating the log2 ratios of spliced to unspliced transcripts in the slc1 mutant compared to the corresponding values in the wild type. Error bars are SDs. The asterisks indicate statistical significance between the wild type and slc1 mutant, as determined by Student’s t-test (* P < 0.05, ** P < 0.01)