Fig. 2

Function of endogenous NO in RBSDV infection. a Endogenous NO accumulation in the RBSDV-inoculated Nipponbare or 15HPO187 plants at various hpi. Ten plants were analyzed as ten biological replicates. The data represented the means ± SD of the ten replicates. Entire experiment repeated 3 times with similar results. b and c Activities of nitrate reductase (NR) and mammalian NO synthase (NOS)-like enzyme in the RBSDV-inoculated plants at various hpi. Ten plants were pooled together as one biological experiment. Images are representative of three independent biological experiments. The data represented the means ± SD of the three replicates. d and e Expressions of OsNIA2 and OsNOA1 in the RBSDV-inoculated plants at various hpi. Five plants were pooled together as one biological experiment. The data represented the means ± SD of the three biological replicates. Entire experiment repeated 3 times with similar results. Different low case letters above the bars indicate the statistical differences between the treatments using the Duncan’s multiple test