Fig. 3

Phenotypes of OsFPFL4 transgenic lines are correlated with OsFPFL4 transcript levels. a Seven-day-old wild type (WT) and OsFPFL4 transgenic seedlings in 1/2 MS agar in tubes. Scale bars, 2 cm. b Analysis of OsFPFL4 transcript levels in 7-day-old seedlings from independent transgenic lines and WT by quantitative real-time PCR. Error bars indicate standard error (n = 3). Three replica experiments were performed. c-g Shoot length, PR length, LR density, AR number per plant and average AR length in 7-day-old WT and transgenic seedlings. Uniformly germinated were grown in 1/2 MS agar medium in tubes for 7 days in a growth chamber. h Root systems of 7-day-old WT and transgenic plants. Scale bars, 1 cm. WT, wild type. OE-5 and OE-9, OsFPFL4 overexpression transgenic lines. RNAi-3 and RNAi-8, OsFPFL4-RNAi transgenic lines. Data are means ± SE (n = 15–30). The significant difference between OsFPFL4 transgenic lines and wild type was determined by Student’s t-test. ^*p < 0.05, ^**p < 0.01