Fig. 6

The genotypes of the F₂ plants at the two LInDel marker loci and the verification of the heteroduplexes. a and b the genotypes of the F₂ plants at the InDel marker loci of LInD1–28 and LInD1–147, three different bands were explained with red arrows. c the verification of the heteroduplexes. Lanes 1–10 are the nondenatured PCR products as follows: lanes 1–3, three F₂ plants; lanes 4–6, ZS97, MH63 and their F₁ cross SY63; lane 7, the mixed PCR products of the two types of homozygous F₂ plants; lane 8, the mixed PCR products of ZS97 and MH63; lane 9, the PCR products amplified from the mixed DNA of two types of homozygous F₂ plants; lane 10, the PCR products amplified from the mixed DNA of ZS97 and MH63; lanes 11–16, the denatured PCR products: lanes 11–12, the heterozygous F₂ plant and SY63; lane 13, the mixed PCR products of the two types of homozygous F₂ plants; lane 14, the mixed PCR products of ZS97 and MH63; lane 15, the PCR products amplified from the mixed DNA of the two types of homozygous F₂ plants; and lane 16, the PCR products amplified from the mixed DNA of ZS97 and MH63