How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis

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Table 1 Web-based tools for sgRNA design

Name	Website	Reference
Benchling	https://www.benchling.com/crispr/	Benchling, CA
Broad Institute GPP	https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design	Doench et al. 2016
CHOPCHOP	http://chopchop.cbu.uib.no/	Labun et al. 2019
CRISPOR	http://crispor.tefor.net/	Concordet and Haeussler 2018
CRISPR-P	http://cbi.hzau.edu.cn/CRISPR2/.	Liu et al. 2017
DeskGen	https://www.deskgen.com/landing/#/	Desktop Genetics, MA
E-CRISP	http://www.e-crisp.org/E-CRISP/designcrispr.html	Heigwer et al. 2014
Horizon Discovery	https://dharmacon.horizondiscovery.com/gene-editing/crispr-cas9/crispr-design-tool/	Horizon Discovery, UK
IDT	https://sg.idtdna.com/site/order/designtool/index/CRISPR_CUSTOM	Integrated DNA Technologies, IA
Off-Spotter	https://cm.jefferson.edu/Off-Spotter/	Pliatsika and Rigoutsos 2015
Synthego	https://www.synthego.com/products/bioinformatics/crispr-design-tool	Synthego, CA
ZiFiT	http://zifit.partners.org/ZiFiT/ChoiceMenu.aspx	Sander et al. 2010