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Table 1 Bacterial strains and plasmids

From: OsPGIP1-Mediated Resistance to Bacterial Leaf Streak in Rice is Beyond Responsive to the Polygalacturonase of Xanthomonas oryzae pv. oryzicola

Strains/plasmids

Characteristics

Source

Strains

 DH5α

Escherichia coli for plasmids transformation

Lab collection

 EHA105

Agrobacterium tumefaciens for rice transformation, RifR

Lab collection

 RS105

Wild type Xoc strain, RifR

Zou et al. 2012

 RS105Δpg

Deletion of XocPG gene in RS105, RifR

This study

 RS105Δpg-CP

XocPG gene complementation in RS105Δpg, KmR, RifR

This study

Plasmids

 pK18mobsacB

Suicide vector for homologous recombination, KmR

Schäfer et al. 1994

 pK18mobsacB-PG

Deletion, upstream and downstream fragments of XocPG cloned in suicide vector pK18mobsacB, KmR

This study

 pVSP61

Expression vector, KmR

Loper and Lindow 1987

 pVSP61-PG

Complementation, XocPG cloned in pVSP61 vector, KmR

This study

 pU1301::OsPGIP1

Rice transformation, OsPGIP1 cloned in constitutive expression vector pU1301, Ubi, KmR

This study

 pDS1301::OsPGIP1

Rice transformation, OsPGIP1 fragment cloned in RNA-silenced vector pDS1301, 35S, KmR

This study

 BD-PG

Yeast two-hybrid, XocPG cloned in the bait vector pGBKT7, KmR

This study

 AD-OsPGIP1

Yeast two-hybrid, OsPGIP1 cloned in the prey vector pGADT7, AmpR

This study

 AD-OsPGIP4

Yeast two-hybrid, OsPGIP4 cloned in the prey vector pGADT7, AmpR

This study

 pGBKT7–53

Yeast two-hybrid, positive bait vector with murine p53, KmR

Clontech

 pGADT7-T

Yeast two-hybrid, positive bait vector with SV40 large T-antigen, AmpR

Clontech