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Table 1 Bacterial strains and plasmids

From: OsPGIP1-Mediated Resistance to Bacterial Leaf Streak in Rice is Beyond Responsive to the Polygalacturonase of Xanthomonas oryzae pv. oryzicola

Strains/plasmids Characteristics Source
Strains
 DH5α Escherichia coli for plasmids transformation Lab collection
 EHA105 Agrobacterium tumefaciens for rice transformation, RifR Lab collection
 RS105 Wild type Xoc strain, RifR Zou et al. 2012
 RS105Δpg Deletion of XocPG gene in RS105, RifR This study
 RS105Δpg-CP XocPG gene complementation in RS105Δpg, KmR, RifR This study
Plasmids
 pK18mobsacB Suicide vector for homologous recombination, KmR Schäfer et al. 1994
 pK18mobsacB-PG Deletion, upstream and downstream fragments of XocPG cloned in suicide vector pK18mobsacB, KmR This study
 pVSP61 Expression vector, KmR Loper and Lindow 1987
 pVSP61-PG Complementation, XocPG cloned in pVSP61 vector, KmR This study
 pU1301::OsPGIP1 Rice transformation, OsPGIP1 cloned in constitutive expression vector pU1301, Ubi, KmR This study
 pDS1301::OsPGIP1 Rice transformation, OsPGIP1 fragment cloned in RNA-silenced vector pDS1301, 35S, KmR This study
 BD-PG Yeast two-hybrid, XocPG cloned in the bait vector pGBKT7, KmR This study
 AD-OsPGIP1 Yeast two-hybrid, OsPGIP1 cloned in the prey vector pGADT7, AmpR This study
 AD-OsPGIP4 Yeast two-hybrid, OsPGIP4 cloned in the prey vector pGADT7, AmpR This study
 pGBKT7–53 Yeast two-hybrid, positive bait vector with murine p53, KmR Clontech
 pGADT7-T Yeast two-hybrid, positive bait vector with SV40 large T-antigen, AmpR Clontech