Fig. 3

The polygalacturonase gene acts as a virulence factor of RS105 during infection in rice. a The position of the polygalacturonase (PG) gene in the Xoc RS105 genome. b The phenotype of RS105 and the PG mutant strain (RS105Δpg) on the susceptible rice variety ZH11 and moderately resistant variety Acc8558. Images were photographed 14 days after inoculation. c The statistical counting of lesion length at 14 days after inoculation with RS105 and RS105Δpg on ZH11 and Acc8558. The data were counted from over 10 plants and analyzed using a t-test (P < 0.05). Asterisks represent statistically significant differences from ZH11. d The bacterial growth curves of RS105 and RS105Δpg in ZH11 and Acc8558 rice at 1, 4, and 7 days. Significant differences were determined by t test: *P < 0.05. e Lesion length after inoculation with RS105, RS105Δpg and PG gene complementary stain (RS105Δpg-CP) at 14 days in ZH11. The letters above the bars represent the significant differences at a value of P ≤ 0.05 (LSD test). The above experiments were repeated three times with similar results