Table 1 Molecular markers used for foreground selection in this study
Marker | Type | Chr. | Forward primer sequence | Reverse primer sequence | Annealing temperature (°C) | Separation |
|---|---|---|---|---|---|---|
RM224 | SSR | 11 | ATCGATCGATCTTCACGAGG | TGCTATAAAAGGCATTCGGG | 56 | 8% PAGE |
Ind306 | Indel | 6 | TGACTTCCAAACGGTAGC | AGAGCTCGTGAACGGAATG | 56 | 8% PAGE |
Pita-Ext | Pita-based marker | 12 | TGCGCAAAGAATCGTCGCTGC | TCTTTGATCCAAGTGTTAGGGCC | 62 | 1% Agarose gel |
Pita-Int | 12 | CCGTGGCTTCTATCTTTACCTG | AGTCAGGTTGAAGATGCATAGA | 62 |