Fig. 5From: CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzaeHomozygous mutants in both Os8N3 alleles displayed enhanced resistance to Xoo. Transgenic Kitaake plants targeting xa13 (OsU6a xa13m/Kit T2) display enhanced resistance to Xoo. a Inoculation results for mutant rice lines 12 DAI with Xoo. From left to right: Kitaake (Kit), transgenic line (XA21, 7A-8) carrying Xa21 driven by the ubiquitin promoter, and transgenic lines (OsU6a xa13m/Kit, T2) carrying the OsU6a::xa13m-sgRNA/pHAtc construct. Arrowheads indicated the end of the lesion. He; Heterozygous; WT; wild type: Ch; chimeric: Ho; homozygous. b Lesion lengths measured 12 DAI in Kitaake, XA21, and OsU6a xa13m/Kit T2. Error bars in the graph represent standard error of at least three leaves from each plant. Letters indicate a significant difference at P < 0.050 by Tukey’s HSD test. c Bacterial population in Kitaake, XA21, and OsU6a xa13m/Kit T2 plants 0 and 12 DAI, determined by the number of CFU per inoculated leaf. Error bars represent standard deviation from at least three technical replicates. Letters indicate a significant difference at P < 0.050 by Tukey’s HSD testBack to article page