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Rice
Fig. 3 | Rice

Fig. 3

From: OsDGD2β is the Sole Digalactosyldiacylglycerol Synthase Gene Highly Expressed in Anther, and its Mutation Confers Male Sterility in Rice

Fig. 3

Targeted mutagenesis of OsDGD2β using CRISPR/Cas9 system. a Two transcripts of OsDGD2β with the target region shown in blue. b DNA sequences of the target region with PAM and genome specific sgRNA sequence shown in red and green, respectively. osdgd2β-1 is a homozygous mutant with 1-bp deletion while osdgd2β-2 is a biallelic mutant with 2-bp (osdgd2β-2a) and 5-bp (osdgd2β-2b) deletion. c schematic representation of truncation in protein translation in the mutants. Purple colour depicts the only domain (Glycos_transf_1), changes in amino acid sequences in the mutants’ protein are shown in brown and yellow colour after the target region

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