Fig. 4

Substituted and mismatched amino acids in wild-type OsPGIP2 and mutated variants OsPGIP2^L233F, OsPGIP2^R253Q, and OsPGIP^Q300H. a, Amino acid sequence of OsPGIP2, OsPGIP2^L233F, OsPGIP2^R253Q, and OsPGIP^Q300H. (i) Signal peptide; (ii), predicted N-terminus of mature proteins; (iii) LRR motifs; and (iv), C-terminus. Black and red rectangles represent targeted and unanticipated mutations, respectively. b, Cartoon model of OsPGIP2. Red and green balls denote mismatched and substituted amino acids, respectively.