Fig. 1

Characterization of the rgb1 mutants generated using CRISPR/Cas9 in rice. a Schematic diagram of the genomic region and the predicted protein structure of RGB1 and the target guide sequences are labeled in black uppercase letters. The structure of the p35S-RGB1_Cas9 vector is shown at the bottom of the panel. b Sequence alignment of the rgb1 mutants showing altered bases compared to the reference genomic RGB1 sequence. The red arrow indicates the cleavage sites. The targeted sequence is highlighted in blue, and the protospacer adjacent motif (PAM) sequences are marked in green and underlined. Indels are indicated by red letters (insertions) or dashes (deleted nucleotides). c Real-time RT-PCR analysis of RGB1 transcripts in the embryos of the WT and rgb1 mutant lines during the early stages of postgermination growth. The PCR signals were normalized to those of actin transcripts. The data are presented as the means±SDs. (n = 3) (d-e) Comparison of seedlings of the rgb1 mutant, RGB1/rgb1 mutant and wild type. d Germinated dehulled seeds growing on filter paper atop wet sand for 5 days after germination. e Germinated seeds growing on plates containing distilled water for 5 days after germination. The white scale bars represent 1 cm