Fig. 5
From: Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9–1
Response of gs9–1 to GA3 and qRT-PCR assays of gs9–1. Asterisks indicate significant differences using the unpaired Student’s t-test (*P < 0.05; ** P ≤ 0.01). a GA3 response assays. Elongation of the second leaf sheath in gs9–1 and Kitaake in response to GA3. b Relative length change of the 2nd leaf sheath, which was calculate by dividing the length of the 2nd leaf sheath stimulated with GA3 by that without GA3. c qRT-PCR assays of four genes involved in GA biosynthesis in the 2nd leaf sheath of gs9–1 and Kitaake. The expression level of each gene from Kitaake was set to 1. The actin gene was used as the internal control. Data are means ± SD (n = 3). d qRT-PCR assays of three genes involved in rice tillering in lateral buds of gs9–1 and Kitaake