Fig. 9
From: A 3-bp deletion of WLS5 gene leads to weak growth and early leaf senescence in rice
Candidate gene analysis for WLS5. a Location of WLS5 on rice chromosome 5. b Coarse linkage map of WLS5. Markers used for mapping are indicated. Numbers below lines indicate number of recombinants. c Candidate genes for WLS5. d LOC_Os05g04900 structure and sequence variations between wild type and wls5. Black and red arrows indicate mutation site and target knockout position, respectively. e, f LOC_Os05g04900 knockout in ‘Nipponbare’ (NPB). CRISPR/Cas9-induced 18-bp deletion in LOC_Os05g04900 (e). Phenotype of NPB and line #20. Bars = 25 cm (left) and 12.5 cm (right) (f)