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Rice
Fig. 5 | Rice

Fig. 5

From: Ethylene signaling involves in seeds germination upon submergence and antioxidant response elicited confers submergence tolerance to rice seedlings

Fig. 5

The detection of O2− and H2O2 accumulation and antioxidative enzyme activity assay in rice seedlings pretreated with an ethylene precursor under submergence conditions. a The NBT and DAB staining methods for detecting the accumulation of O2− and H2O2 in detached leaves of 8-day-old TK9 rice seedlings treated under Nor, Sub, and Pre ACC + Sub for 2 days and then subjected to submergence for 4 days. Same results were obtained in three independent experiments. Photograph exhibits results of three independent leaves. Bar = 1 cm. b The enzyme activity of detached leaves of 8-day-old TK9 rice seedlings treated under Nor, Sub, and Pre ACC + Sub for 2 days and then subjected to submergence for 4 days. CAT, APX, SOD, and POX activities are the average values ± standard deviation of three biologically independent experiments each with duplicate samples. The values with different letters are significantly different at P < 0.05, according to a one-way ANOVA with post hoc Duncan’s test

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