Fig. 3

Sequence analysis of the cellusase LSSR1. The underlined 19 amino acids in the 5′ terminal are predicted as a signal peptide. The position of the β-strands of the (β/α)₈ barrel are indicated by arrows above the alignment based on template c2zunB. Amino acids in yellow indicate the binding site, amino acids in red indicate the catalytic site, and the blue E (glutamic acid) may have both binding activity and catalytic activity. The blue E and the red E in β-strands 4 and 7 are the acid/base and nucleophile, respectively. The arrowed W²¹⁶ (tryptophan) is the site where mutations in L1 lines begin