Fig. 1

The CRISPR/Cas9 target site of TH1 and phenotype of the KO plants. a Schematic of TH1 gene structure and the CRISPR/Cas9 target site. TH1 contains a single exon indicated by gray rectangles. The translation initiation codon (ATG) and termination codon (TGA) are shown. The target site nucleotides are shown in capital letters, and the protospacer adjacent motif (PAM) site is underlined. b, c Panicles of wild type and KO plant. d-f spikelet of wild type and KO plant. g, h Pollen viability test for wild type and KO plant, represents the pollen activity for 3 biological replicates of each line respectively. i Statistical data of pollen activity. Values are shown as means ± s.d. (n = 3). P values were calculated by the Student’s t-test. Scale bar is 5 cm in (b) and (c), 2 mm in (d), e and (f), 200 μm in (g) and (h)