Fig. 3
From: The ATP-binding cassette (ABC) transporter OsABCG3 is essential for pollen development in rice
Expression pattern analysis of OsABCG3 and subcellular localization analysis of OsABCG3 protein. a Expression pattern of OsABCG3. Anthers were collected at different developmental stages (St 6–12). Pistils and other tissues were harvested from plants at heading stage. The expression levels were determined by qRT-PCR. OsACTIN1 was used as internal standards. Data are shown as means ± SD (n = 3). b-c GUS staining of the OsABCG3pro: GUS transgenic plant. The spikelets with the palea and lemma removed (b), and cleared mounted anther and microspores (c) were showed respectively. d In situ hybridization of OsABCG3 antisense and sense probes with the WT anther sections, respectively. e, f Subcellular localization of OsABCG3-GFP fusion protein. The fusion protein GFP-OsABCG3 was co-expressed with mCherry-OsRac3, a plasma membrane marker, by transient expression in rice protoplasts (e). The construct 35S:GFP was transformed as a control (f). Localization of the fluorescence-tagged proteins was examined by confocal microscopy. Scale bars =1 mm (b); 25 μm (c, d); 5 μm (e, f)