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Rice
Fig. 9 | Rice

Fig. 9

From: DS1/OsEMF1 interacts with OsARF11 to control rice architecture by regulation of brassinosteroid signaling

Fig. 9

DS1 interacts with OsARF11 to promote the expression of OsBRI1. a Yeast one-hybrid (Y1H) analysis of DS1, OsARF11 and OsBRI1 promoter. The bait vector containing the OsBRI1 promoter fragment-fused lacZ reporter gene, and the prey vector containing DS1/OsARF11-fused GAL1 activation domain were co-transformed into yeast cells (EGY48). b Transient expression assays of OsBRI1 transcriptional activity modulated by DS1/ds1 and OsARF11 in rice protoplasts. Various constructs used in transient expression assays are shown in the upper panel. pOsBRI1:LUC was cotransformed with either the effector or empty vector (control) into rice protoplasts. Relative LUC activity indicating the level of OsBRI1 expression activated by the effector is shown in the lower panel. Values are means ± SD of three biological replicates. c Model representing mechanism of action of DS1 in regulating OsBRI1

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