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Rice
Fig. 5 | Rice

Fig. 5

From: Overexpression of OsbHLH107, a member of the basic helix-loop-helix transcription factor family, enhances grain size in rice (Oryza sativa L.)

Fig. 5

Expression patterns, subcellular-localization, and transcriptional activity analysis of OsbHLH107. a Relative expression levels of OsbHLH107 in various developmental stages of the panicles. P, panicles. OsActin1 was used as the internal control (n = 3). Data are the mean ± SD. b The OsbHLH107-GFP fusion protein was located in the nucleus in rice protoplasts. Scale bar, 20 μm. c Transcriptional activity assays of OsbHLH107 and its series of domain deletion variants in the yeast GAL4 system. Numbers below the schematic represent amino acid positions of OsbHLH107. DNA-BD represents the GAL4 DNA-binding domain. Empty pGBKT7 vector was used as the negative control

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