Skip to main content
Rice
Fig. 3 | Rice

Fig. 3

From: Overexpression of OsbHLH107, a member of the basic helix-loop-helix transcription factor family, enhances grain size in rice (Oryza sativa L.)

Fig. 3

Identification of the T-DNA insertion site in lgs1. a OsbHLH107 (LOC_Os02g56140) located at the end of rice chromosome 2. Black lines, black rectangles, and white rectangles in the genomic region represent introns, exons, and UTRs, respectively. b The T-DNA insertion site in lgs1. ATG and TAA represent the start and stop codons in the CDS, respectively. The four copies of 35S enhancers in the T-DNA insertion sequence are indicated with green arrows. RB and LB indicate the T-DNA vector right and left borders, respectively. P1, P2, and P3 are primers used for identifying the T-DNA insertion site. Qrt1, Qrt2, and Qrt3 are primer pairs used for qRT-PCR analysis of different regions of LOC_Os02g56140. c Identification of the T-DNA insertion by PCR analysis. d qRT-PCR analysis of different regions of LOC_Os02g56140 and the genes upstream or downstream of it, with RNA isolated from leaves. OsActin1 was used as the internal control, and the relative expression levels in the WT were set to one (n = 3). Data are the mean ± SD

Back to article page