Fig. 2

Increased cell number of spikelet hulls in the longitudinal direction determines lgs1 phenotype. a Young spikelet hulls of ‘Dongjin’ (WT) and lgs1. Scale bar, 5 mm. b Electron microscopic observation of inner epidermal cells. Scale bar, 100 μm. c-e Total length (c, n = 10), cell length (d, n = 100), and cell number (e, n = 10) of inner epidermal cells. f Relative expression levels of genes associated with the cell cycle. Relative expression levels of each gene were determined by qRT-PCR with RNA isolated from 5 cm panicles of WT and lgs1. OsActin1 was used as the internal control, and the values of the relative expression levels in the WT were set to one (n = 3). Data are the mean ± SD. Student’s t-tests were used to generate the P values. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001